KMID : 0352720210450050610
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Journal of Ginseng Research 2021 Volume.45 No. 5 p.610 ~ p.616
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Ginsenoside Rg3 attenuates skin disorders via down-regulation of MDM2/HIF1¥á signaling pathway
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Han Na-Ra
Ko Seong-Gyu Moon Phil-Dong Park Hi-Joon
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Abstract
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Background: Thymic stromal lymphopoietin (TSLP) acts as a master switch for inflammatory responses. Ginsenoside Rg3 (Rg3) which is an active ingredient of Panax ginseng Meyer (Araliaceae) is known to possess various therapeutic effects. However, a modulatory effect of Rg3 on TSLP expression in the inflammatory responses remains poorly understood.
Methods: We investigated antiinflammatory effects of Rg3 on an in vitro model using HMC-1 cells stimulated by PMA plus calcium ionophore (PMACI), as well as an in vivo model using PMA-induced mouse ear edema. TSLP and vascular endothelial growth factor (VEGF) levels were detected using enzyme-linked immunosorbent assay or real-time PCR analysis. Murine double minute 2 (MDM2) and hypoxia-inducible factor 1¥á (HIF1¥á) expression levels were detected using Western blot analysis.
Results: Rg3 treatment restrained the production and mRNA expression levels of TSLP and VEGF in activated HMC-1 cells. Rg3 down-regulated the MDM2 expression level increased by PMACI stimulation. The HIF1¥á expression level was also reduced by Rg3 in activated HMC-1 cells. In addition, Rg3-administered mice showed the decreased redness and ear thickness in PMA-irritated ear edema. Rg3 inhibited the TSLP and VEGF levels in the serum and ear tissue homogenate. Moreover, the MDM2 and HIF1¥á expression levels in the ear tissue homogenate were suppressed by Rg3.
Conclusion: Taken together, the current study identifies new mechanistic evidence about MDM2/HIF1¥á pathway in the antiinflammatory effect of Rg3, providing a new effective therapeutic strategy for the treatment of skin inflammatory diseases.
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KEYWORD
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Ginsenoside Rg3, Thymic stromal lymphopoietin, Murine double minute 2, Hypoxia-inducible factor 1¥á, Ear edema
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